Carbohydrate-binding specificity of the daffodil (Narcissus pseudonarcissus) and amaryllis (Hippeastrum hybr.) bulb lectins

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Carbohydrate-binding specificity of the daffodil (Narcissus pseudonarcissus) and amaryllis (Hippeastrum hybr.) bulb lectins.

The carbohydrate binding specificity of the daffodil (Narcissus pseudonarcissus; NPA) and amaryllis (Hippeastrum hybr.; HHA) lectins, isolated from extracts of their bulbs by affinity chromatography on immobilized mannose, was studied by quantitative precipitation, sugar hapten inhibition, and affinity chromatography on the immobilized lectins. These lectins gave strong precipitation reactions ...

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Apparent Identity of Chromoplast and Chloroplast DNA in the Daffodil, Narcissus pseudonarcissus

Although chromoplasts and chloroplasts are mor­ phologically and functionally very different, it has been shown in certain plants and under certain con­ ditions, that they are interconvertible [see 1 5]. One could then expect such plastids to contain, at least qualitatively, the same plastome. It appears questionable, however, whether this applies to all plastid types. For example, FreyWyssling...

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Identification of genes associated with perianth senescence in Daffodil (Narcissus pseudonarcissus

We used a PCR-based subtractive procedure to isolate genes that increased in abundance in the perianth of incipiently senescent daffodil (Narcissus pseudonarcissus L. ‘Dutch Master’) flowers. Using RNA gel blot analysis we confirmed that 18 of the transcripts were senescence-associated. A number of these have homologs in previously-studied senescing floral and leaf tissue systems (cysteine prot...

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Lectins as Carbohydrate-binding Proteins

Lectins are cell-binding and agglutinating (glyco)proteins found in a variety of living forms. They will be defined here as homogeneous carbohydrate-binding (glyco)proteins principally of plant (seed) origin but also occurring in invertebrate (and vertebrate?) forms. In recent years, lectins have been employed in studying a wide range of biological phenomena including cell differentiation and m...

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The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectins.

The carbohydrate-binding specificity of lectins from the seeds of Canavalia maritima and Dioclea grandiflora was studied by hapten-inhibition of haemagglutination using various sugars and sugar derivatives as inhibitors, including N-acetylneuraminic acid and N-acetylmuramic acid. Despite some discrepancies, both lectins exhibited a very similar carbohydrate-binding specificity as previously rep...

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ژورنال

عنوان ژورنال: Archives of Biochemistry and Biophysics

سال: 1990

ISSN: 0003-9861

DOI: 10.1016/0003-9861(90)90495-k